imported>Lcovelli at 13:10, 25 May 2010

2010-05-25T13:10:09Z

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{{Element
|genbank=[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=125860391 125860391]
|type=
|genus= Badnavirus
|subfamily= Class 2
|family= Caulimoviridae
|group= LTR retroelements
|description=Yam (''Dioscorea'' spp.),for their large underground tubers and aerial bulbils, is an important food in tropical and sub-tropical regions, particularly in West Africa and the South Pacific. A typical symptomatology, known as "internal brown spot" affecting tubers Yam, was found to be potentially associated to the presence of badnavirus-like particles ([[Literature:100637|Harrison and Roberts 1973]]). Although several sequences have been identified in various ''Dioscorea'' species ([[Literature:100639|Kenyon'' et al.'' 2008]]; [[Literature:86893|Bousalem ''et al.'' 2009]]), to date only two -''Dioscorea alata bacilliform virus'' (DaBV) and ''Dioscorea sansibarensis bacilliform virus'' (DsBV)- have been entirely characterized ([[Literature:100610|Briddon ''et al.'' 1999]]; [[Literature:97182|Seal and Muller 2007]]). The DBV sequence selected for our study is that isolated from yam ''Dioscorea sansibarensis'' species (DsBV). It is a member of the plant pararetrovirus genus ''Badnavirus'' of ''Caulimoviridae'' family (''International Committee on the Taxonomy of Viruses'' -ICTV- [[Literature:100589|Fauquet ''et al''. 2005]]). According to [[Literature:100596|Llorens ''et al''. 2009]], DBV belongs to ''Badnavirus'' genus within Class 2 of the [[Caulimoviridae|''Caulimoviridae'']] family.

DsBV bacilliform particles of approximately 130- 30 nm in size, contain a circular double-stranded DNA genome 7261 bp long encoding for three Open reading frames (ORFs I, II, III) ([[Literature:97182|Seal and Muller 2007]]). The putative ORF I protein is similar to those of the correspondent ORFs encoded by badnaviruses and showing sequence similarity to the ''Caulimoviridae'' virion associated proteins (VAPs). ORF II potentially encodes a protein of 14.1 kDa which function is still unknown. The amino acid sequence of ORF III contains the characteristic MOV, COAT, PR, RT and RH domains, including the large cysteine-rich region (only observed in Badna- and Tungroviruses) at the C-terminus of the COAT (''gag'') domain ([[Literature:97182|Seal and Muller 2007]]; [[Literature:100596|Llorens ''et al''. 2009]]; [[Literature:100605|Hull 1996]]; [[Literature:8054|Bouhida ''et al.'' 1993]]). Interestingly, the DBV ORF III-polyprotein contains an additional dUTPase domain upstream to the COAT domain similar to that of several ''Retroviridae'' retroviruses ([[Literature:20174|Elder ''et al''. 1992]]) and ''Ty3/Gypsy'' LTR retrotransposons ([[Literature:95064|Novikova and Blinov 2008]]). A similar dUTPase domain has also been found in the genome of other ''Badnavirus'' species, ''Taro bacilliform virus'' (TaBV).

DBV is transmitted mechanically, as well as by the mealybug ''Planococcus citri'' from ''Dioscorea alata'' to some other ''Dioscorea'' species but, due to its low titer accumulation in yams, sometimes does not produce pronounced symptoms on leaves that only show mild deformation and veinal chlorosis ([[Literature:100638|Phillips ''et al.'' 1999]]; [[Literature:97182|Seal and Muller 2007]]). As flowering and seed-setting is rare, yam plants are usually vegetatively propagated by planting of small tubers or tuber pieces ([[Literature:100639|Kenyon ''et al.'' 2008]]). The greatest risk of virus spread is that caused by vegetative propagation of infected material.
Badnavirus-like particles, first reported in yams in the 1970s, were associated with a flexuous virus presents in ''D. alata'' and ''D. cayenensis'' species with "internal brown spot disease" ([[Literature:100637|Harrison and Roberts 1973]]). Subsequently, serologically related bacilliform badnavirus particles have been detected in several other ''Dioscorea'' species of different geographical regions worldwide ([[Literature:100639|Kenyon ''et al.'' 2008]]; [[Literature:97182|Seal and Muller 2007]]; [[Literature:100638|Phillips ''et al.'' 1999]]; [[Literature:100610|Briddon ''et al.'' 1999]]). [[Literature:100639|Kenyon ''et al.'' (2008)]] and [[Literature:86893|Bousalem ''et al.'' (2009)]], show the existence of different ''Badnavirus''-like species highly diverse and prevalent in yams of different geographical areas, some of which represent previously uncharacterised and unnamed species. In particular, three sequences (two groups) have been found highly divergent to the other new South Pacific yam ''Badnavirus'' groups, so has been supposed probably they represent either new ''Caulimoviridae'' genera or endogenous pararetrovirus sequences ([[Literature:100639|Kenyon ''et al.'' 2008]]). Although the integration of badnavirus sequences into the genome of ''D. rotundata'' has been suggested, further analyses should be necessary to determine if these sequences are dead integrants or activatable sequences ([[Literature:86893|Bousalem ''et al.'' 2009]]). Unfortunately to date, the lack of any sufficiently specific antisera to yam badnaviruses hinders to differentiate between viral episomal and integrated sequences ([[Literature:86893|Bousalem ''et al.'' 2009]]).
|structure=DBV.png
|host=Dioscorea sansibarensis
|hostpic=Dioscorea.jpg
|literature=
|picowner=[http://www.hear.org/starr/images/600/starr-031108-0225.jpg Courtesy of Forest & Kim Starr, Hawaiian Ecosystems at Risk project (HEAR)]
}}
[[Category:Element]]

Element:DBV - Revision history

imported>Lcovelli at 13:10, 25 May 2010