imported>Gydbwiki at 12:19, 29 January 2010

2010-01-29T12:19:47Z

New page

{{Element
|genbank=[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=25992566 AF533704]<br> [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=25992566 AF533703 (LTRs)]
|type=
Pyggy
|genus=
Fungi and vertebrates
|subfamily=
Chromoviruses
|family=
Ty3/Gypsy
|group=
LTR retroelements
|description=''Pyggy'' is a LTR retrotransposon described in ''Pyrenophora graminea'' ([[Literature:85489|Konstantinova et al. 2002]]). ''Pyggy'' belongs to the ''Chromoviridae'' branch, term suggested ([[Literature:47259|Marín and Lloréns 2000]]) to describe the ''Ty3/Gypsy'' elements bearing chromodomain-integrases ([[Literature:46552|Malik and Eickbush 1999]]). The chromovirus branch is probably the most ancient phylogenetic pattern of ''Ty3/Gypsy'' retroelements ([[Literature:26737|Gorinsek, Gubensek and Kordis 2004]]; [[Literature:26736| 2005]]; [[Literature:39523|Kordis 2005]]).

To date, the available sequence of ''Pyggy'' is a degenerated clone (accession AF533704) whose genome is 6.1 Kb in size, including LTRs of 224 nt (accession AF533703). The internal region of this element displays a Primer Binding site (PBS), two pseudogenized Open Reading Frames (ORFs) for ''gag'', and ''pol'' genes, and a Polypurine Tract (PPT) adjacent to the 3' LTR ([[Literature:85489|Konstantinova et al. 2002]]). This element presents a detectable chromodomain at the C-terminal end of the integrase domain. The PBS of fungi and vertebrate chromoviruses, differs significantly from that used by plant chromoviruses. While plant chromoviruses use a methionine starting tRNA (iMet), fungi and vertebrate chromoviruses use their own self-priming mechanism to start the reverse transcription ([[Literature:43217|Levin 1995 ]]; [[Literature:10125|Butler et al. 2001]]).
|structure=Pyggy.png
|host=Pyrenophora graminea
|hostpic=Pyrenophora_graminea.gif
|literature=*[[Literature:15303|Mobile DNA II. (Craig et al. 2002)]]
|picowner=[http://www.skinet.be/Champignons_passion/gal_mi2.htm Image, courtesy of Marcel Lecomte, Skinet.be]
}}

Element:Pyggy - Revision history

imported>Gydbwiki at 12:19, 29 January 2010