imported>Lcovelli at 10:14, 19 April 2010

2010-04-19T10:14:30Z

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{{Element
|genbank=[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=221048143 221048143]
|type=
|genus=Caulimovirus
|subfamily=Class 1
|family=Caulimoviridae
|group=LTR retroelements
|description=''Rudbeckia flower distortion virus'' (RuFDV) is an unclassified plant pararetrovirus found in ''Rudbeckia'' ornamental plants species (a genus of herbaceous perennial plants belonging to ''Asteraceae'' family) and proposed to be a tentative new ''Caulimovirus'' member ([http://www.ncbi.nlm.nih.gov/nuccore/221048143 Lockhart and Olszewsk 2009], unpublished) of the ''Caulimoviridae'' family (''International Committee on the Taxonomy of Viruses'' -ICTV- [[Literature:100589|Fauquet ''et al''. 2005]]).This is a perspective clearly supported by phylogenetic analyses inferred based on the COAT(GAG) and pol protein domains ([[Literature:100596|Llorens ''et al'' 2009]]).

The RuFDV virion encapsidates a genomic circular double-stranded DNA molecule of 8222 bp long. The genome encode for seven Open reading frames (ORFs) designated as gp1, gp2, gp3, gp4, gp5, gp6, gp7 whose sequence, size, and organization are similar to those of caulimoviruses ([http://www.ncbi.nlm.nih.gov/nuccore/221048143 Lockhart and Olszewsk 2009], unpublished). Our sequence analyses show that ORF-gp3 does not reveal significant similarities with the "virion associated protein" (VAP)-ORF III of the other ''Caulimoviridae'' species. It is more similar to those of the soymoviruses ORFs C (SbCMV, BRRV and CmYLCV) whose function is still unclear. The sequences of the other protein products show similarities with those encoded by the corresponding ORFs of known caulimoviruses. ORF-gp2 encodes the putative movement protein (MOV), while ORF-gp4 contains the "RNA-binding" motif characteristic of the ''Caulimoviridae'' COAT(''gag'')-like domain (C-X-C-X2-C-X4-H-X4-C ) ([[Literature:100605|Hull 1996]]; [[Literature:8054|Bouhida ''et al.'' 1993]]; [[Literature:100596|Llorens ''et al'' 2009]]). ORF-gp5 product is a pol polyprotein displaying the typical -aspartic protease (PR), reverse transcriptase (RT) and RNase H (RH) domains, and ORF-gp6 encodes for a translational transactivator protein (TAV) ([http://www.ncbi.nlm.nih.gov/nuccore/221048143 Lockhart and Olszewsk 2009], unpublished). ORF-gp1 and –gp7 functions remain unknown. ORF-gp6 (TAV) and ORF-gp7 are separated from an intergenic region (850 bp long) as also observed in the genome of several soymoviruses such as SbCMV. In contrast to the other Caulimoviruses, between the ORFs-gp2 (MOV) and -gp3 the genome of RuFDV apparently does not encode for any ORF associated to the "aphid transmission factor" (ATF).
|structure=RuFDV.png
|host=Rudbeckia hirta
|hostpic=Rudbeckia_hirta.jpg
|literature=
|picowner=[http://upload.wikimedia.org/wikipedia/commons/4/49/Rudbeckia_hirta.JPG Wikipedia, courtesy of "Greschtester"]
}}
[[Category:Element]]

Element:RuFDV - Revision history

imported>Lcovelli at 10:14, 19 April 2010