https://gydb.org/index.php?action=history&feed=atom&title=Element%3ATy1B 2026-06-09T18:14:40Z Revision history for this page on the wiki MediaWiki 1.35.0 https://gydb.org/index.php?title=Element:Ty1B&diff=643&oldid=prev 2010-01-27T16:38:01Z

<p></p> <p><b>New page</b></p><div>{{Element<br /> |genbank=[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&amp;val=535983 535983 (nts 4201-9469)]<br /> |type= Ty (Pseudovirus)<br /> |genus=Pseudovirus<br /> |subfamily=Branch 1<br /> |family= Ty1/Copia<br /> |group= LTR retroelements<br /> |description=''Ty1'' is representative of one of the four sets of LTR retrotransposons (''Ty1'','' Ty2'', ''Ty3'' and ''Ty4'') described in the genome of yeast ''Saccharomyces cerevisiae'' ([[Literature:100662|Boeke ''et al.'' 1988]]). ''Ty1'' is the largest set and seems to exhibit the highest degree of variation ([[Literature:100661|Boeke and Sandmeyer 1991]]). ''Ty1B'' element has been take as reference sequence to our studies. ''Ty1B'' belongs to ''Ty (Pseudovirus)'' clade one of the three original genera on which the in-progrees ''Pseudoviridae'' classification is constituted ([[Literature:100591|Boeke ''et al'' 2005]]).<br /> <br /> ''Ty1B'' genome is about of 5.9 Kb in size (5983 bp long) and includes a 334-bp 5’LTR and a 332-bp 3’LTR. The structure of this element contains a Primer Binding Site (PBS) complementary to a tRNA&lt;sup&gt;iMet&lt;/sup&gt;, an internal region and two Polypurine Tracts (PPTs): one upstream to the 3’LTR (PPT1) and a second within the pol gene (PPT2)([[Literature:100661|Boeke and Sandmeyer 1991]]). The internal region is composed by two overlapping Open Reading Frames (ORFs) that contain respectively the gag protein (the first ORF) and the typical structure of the ''Ty1/Copia'' pol polyprotein domains: protease, integrase, reverse transcriptase and ribonuclease H (the second larger ORF) ([[Literature:100661|Boeke and Sandmeyer 1991]]). The gag-associated RNA-binding motif (CCHC) has not been identified in this element ([[Literature:59075|Peterson-Burch and Voytas 2002]]). The central PPT2 has been demonstrated to be not essential for the element transposition ([[Literature:30799|Heyman ''et al.'' 2003]]; [[Literature:23281|Friant ''et al.'' 1996]]). <br /> |structure=Ty1B.png<br /> |host=Saccharomyces cerevisiae<br /> |hostpic=Saccharomyces_cerevisiae.gif<br /> |literature=*[[Literature:15303|Mobile DNA II. (Craig et al. 2002)]]<br /> |picowner=[http://www.biotechvana.com Image, Joaquin Panadero, &lt;br&gt;Copyright, GyDB, Biotech Vana]<br /> }}<br /> [[Category:Element]]</div>

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