imported>Gydbwiki at 16:39, 27 January 2010

2010-01-27T16:39:28Z

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{{Element
|genbank=[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=2636718 2636718]
|type= Ty (Pseudovirus)
|genus=Pseudovirus
|subfamily=Branch 1
|family= Ty1/Copia
|group= LTR retroelements
|description= ''pCal'' is a ''Ty1/Copia''-like LTR retrotransposon characterized in the genome of the pathogenic yeast ''Candida albicans'' ([[Literature:48474|Matthews ''et al.'' 1997]]). ''pCal'' element belongs to ''Ty (Pseudovirus)'' clade one of the three original genera on which the in-progrees ''Pseudoviridae'' classification is constituted ([[Literature:100591|Boeke ''et al'' 2005]]).

The genome of ''pCal'' is 6426 bp in size, including two identical 280-bp LTRs that bound an internal coding region containing a Primer Binding Site (PBS), complementary to a tRNA<sup>Arg</sup>, a long Open Reading Frame (ORF) interrupted by one stop codon, and a Polypurine Tract upstream to the 3’LTR (PPT1) ([[Literature:48474|Matthews ''et al.'' 1997]]). Interestingly, a second sequence very similar to the 3’ PPT1 was found near the middle of ''pCal'' (bases 3455 to 3465), it probably serves as a site for plus-strand initiation during the reverse transcription process and to speed up this process ([[Literature:48474|Matthews ''et al.'' 1997]]). The long ORF contains both gag and pol domain structures characteristic of ''Ty1/Copia'' retroelements: gag, protease, integrase, reverse transcriptase and ribonuclease H. The gag-associated RNA-binding motif (CCHC) has not been identified in this element ([[Literature:48474|Matthews ''et al.'' 1997]]).
|structure=pCal.png
|host=Candida albicans
|hostpic=Candida.jpg
|literature=*[[Literature:15303|Mobile DNA II. (Craig ''et al.'' 2002)]]
|picowner=[http://www.wardelab.com/ Courtesy of Dr. Dan Wiedbrauk, "Warde Medical Laboratory" (Ann Arbor, Michigan)]
}}
[[Category:Element]]

Element:pCal - Revision history

imported>Gydbwiki at 16:39, 27 January 2010